Detailed view for Tb927.11.5970
Basic information
Trypanosoma brucei, phosphoinositide-specific phospholipase C
Source Database / ID: TriTrypDB GeneDB
pI: 7.4881 |
Length (AA): 714 |
MW (Da): 80449 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Network
Pfam domains
Gene Ontology
GO:0016020 membrane
GO:0008081 phosphoric diester hydrolase activity
GO:0005509 calcium ion binding
GO:0004629 phospholipase C activity
GO:0004435 phosphoinositide phospholipase C activity
GO:0007242 intracellular signaling cascade
GO:0007165 signal transduction
GO:0006629 lipid metabolic process
Metabolic Pathways
Structural information
Modbase 3D models:
There are 5 models calculated for this protein. More info on
these models, including the
models themselves is available at:
Modbase
| Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
|---|---|---|---|---|---|---|---|---|---|
| 60 | 712 | 3qr1 (A) | 118 | 768 | 28.00 | 0 | 1 | 1.14157 | 0.11 |
| 104 | 166 | 2kff (A) | 56 | 115 | 13.00 | 0 | 0.3 | 0.305235 | -1.54 |
| 107 | 147 | 1oqp (A) | 110 | 148 | 41.00 | 0.077 | 0.97 | 0.568923 | -0.61 |
| 115 | 714 | 1djx (B) | 160 | 755 | 37.00 | 0 | 1 | 1.16034 | 0.08 |
| 590 | 697 | 3fdw (A) | 376 | 483 | 16.00 | 0.092 | 0.02 | 0.141661 | 0.91 |
Help me make sense of these data.
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
Expression
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Upper 80-100% percentile | Procyclic. | Siegel TN |
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Mid 40-60% percentile | Bloodstream Form. | Siegel TN |
-
Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.
Orthologs
Ortholog group members (OG5_127346)
| Species | Accession | Gene Product |
|---|---|---|
| Arabidopsis thaliana | AT5G58670 | phosphoinositide phospholipase C 1 |
| Arabidopsis thaliana | AT5G58700 | phosphoinositide phospholipase C 4 |
| Arabidopsis thaliana | AT2G40116 | phosphoinositide phospholipase C 6 |
| Arabidopsis thaliana | AT3G55940 | phosphoinositide phospholipase C 7 |
| Arabidopsis thaliana | AT3G08510 | phosphoinositide phospholipase C 2 |
| Arabidopsis thaliana | AT5G58690 | phosphoinositide phospholipase C 5 |
| Arabidopsis thaliana | AT4G38530 | putative phosphoinositide phospholipase C 3 |
| Babesia bovis | BBOV_IV011070 | phospholipase C gamma 1, putative |
| Brugia malayi | Bm1_31705 | Phosphatidylinositol-specific phospholipase C, X domain containing protein |
| Candida albicans | CaO19_5506 | hypothetical protein |
| Candida albicans | CaO19.5506 | phospholipase C |
| Caenorhabditis elegans | CELE_R05G6.8 | Protein PLC-4 |
| Cryptosporidium hominis | Chro.40288 | phospholipase C, delta |
| Cryptosporidium parvum | cgd4_2560 | phospholipase C, delta 1 ortholog with 2 EF hands plus phospholipase C domain plus C2 domain |
| Dictyostelium discoideum | DDB_G0292736 | phosphoinositide-specific phospholipase C |
| Homo sapiens | 113026 | phospholipase C, delta 3 |
| Homo sapiens | ENSG00000115556 | phospholipase C, delta 4 |
| Homo sapiens | ENSG00000187091 | phospholipase C, delta 1 |
| Leishmania braziliensis | LbrM.22.1590 | phosphoinositide-specific phospholipase C, putative |
| Leishmania donovani | LdBPK_302970.1 | phospholipase c-like protein |
| Leishmania donovani | LdBPK_221530.1 | phosphoinositide-specific phospholipase C, putative |
| Leishmania infantum | LinJ.22.1530 | phosphoinositide-specific phospholipase C, putative |
| Leishmania infantum | LinJ.30.2970 | phospholipase c-like protein |
| Leishmania major | LmjF.30.2950 | phospholipase c-like protein |
| Leishmania major | LmjF.22.1680 | phosphoinositide-specific phospholipase C, putative |
| Leishmania mexicana | LmxM.29.2950 | phospholipase c-like protein |
| Loa Loa (eye worm) | LOAG_02789 | phosphatidylinositol-specific phospholipase C |
| Mus musculus | ENSMUSG00000026173 | phospholipase C, delta 4 |
| Mus musculus | ENSMUSG00000010660 | phospholipase C, delta 1 |
| Mus musculus | ENSMUSG00000020937 | phospholipase C, delta 3 |
| Neospora caninum | NCLIV_064970 | Phospholipid phospholipase C beta isoform, related |
| Oryza sativa | 4344391 | Os07g0694000 |
| Oryza sativa | 4332497 | Os03g0289300 |
| Oryza sativa | 4337686 | Os05g0127200 |
| Onchocerca volvulus | OVOC9265 |
|
| Onchocerca volvulus | OVOC4175 |
|
| Saccharomyces cerevisiae | YPL268W | phosphatidylinositol phospholipase C |
| Trypanosoma brucei gambiense | Tbg972.11.6720 | phosphoinositide-specific phospholipase C, putative |
| Trypanosoma brucei | Tb927.11.5970 | phosphoinositide-specific phospholipase C |
| Trypanosoma congolense | TcIL3000.11.6490 | phosphoinositide-specific phospholipase C, putative |
| Trypanosoma cruzi | TcCLB.504149.160 | phosphoinositide-specific phospholipase C, putative |
| Theileria parva | TP01_0775 | phospholipase C, putative |
Essentiality
| Gene/Ortholog | Organism | Phenotype | Source Study |
|---|---|---|---|
| Tb11.02.3780 this record | Trypanosoma brucei | significant gain of fitness in bloodstream forms (3 days) | alsford |
| Tb11.02.3780 this record | Trypanosoma brucei | no significant loss or gain of fitness in bloodstream forms (6 days) | alsford |
| Tb11.02.3780 this record | Trypanosoma brucei | significant gain of fitness in procyclic forms | alsford |
| Tb11.02.3780 this record | Trypanosoma brucei | significant gain of fitness in differentiation of procyclic to bloodstream forms | alsford |
| YPL268W | Saccharomyces cerevisiae | inviable | yeastgenome |
-
gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84 -
alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924 -
neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs -
nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource -
yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database -
wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170 -
keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection -
blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930 -
shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
Phenotypes and Validation (curated)
Annotated phenotypes:
| Affected Entity | Phenotypic quality | Occurs in | Occurs at | Evidence | Observed in | Drugs/Inhibitors |
|---|---|---|---|---|---|---|
| cell proliferation (GO:0008283) | increased (PATO:0000470) | bloodstream stage trypomastigotes (PLO:0027) | inferred from RNAi experiment (ECO:0000019) | No drug identifiers listed for this gene. | ||
| Annotator: | fernan@iib.unsam.edu.ar. | Comment: | increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 3 days). | References: | 21363968 | |
| cell proliferation (GO:0008283) | normal (PATO:0000461) | bloodstream stage trypomastigotes (PLO:0027) | inferred from RNAi experiment (ECO:0000019) | No drug identifiers listed for this gene. | ||
| Annotator: | fernan@iib.unsam.edu.ar. | Comment: | normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). | References: | 21363968 | |
| cell proliferation (GO:0008283) | increased (PATO:0000470) | procyclic (PLO:0034) | inferred from RNAi experiment (ECO:0000019) | No drug identifiers listed for this gene. | ||
| Annotator: | fernan@iib.unsam.edu.ar. | Comment: | increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . | References: | 21363968 | |
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Annotated validation
Associated compounds / Druggability
Druggability index (range: 0 to 1): 0.3
Known modulators for this target
Predicted associations
By orthology with druggable targets
| Species | Known druggable target | Linked compounds | Reference |
|---|---|---|---|
| Rattus norvegicus | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 | Compounds | References |
| Homo sapiens | phospholipase C, delta 1 | Compounds | References |
By sequence similarity to non orthologous druggable targets
Obtained from network model
Assayability
Assay information
Reagent availability
Bibliographic References
1 literature reference was collected for this gene.